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crispr cas9 system  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology crispr cas9 system
    Crispr Cas9 System, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/crispr+cas9+ko+plasmid/Heme+Oxygenase+1+CRISPR%2FCas9+KO+Plasmid/pm41934679-54-4-12
    Average 94 stars, based on 4 article reviews
    crispr cas9 system - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    CRISPR:

    Article Title: Compounds, compositions, methods of using, and methods for preparing compounds
    Article Snippet: .. At 60% confluency cells, cell were co-transfected with 2 μg each of CRISPR/Cas9 KO Plasmid (sc-400511-KO-2; Santa cruz) and HDR Plasmid (sc-400511-HDR-2; Santa cruz) using UltraCruz® Transfection Reagent (sc-395739; Santa Cruz). .. The double positive cells for GFP and RFP were sorted using MoFlo XDP sorting instrument (Beckman Coulter).

    Article Title: DARPP-32 is a novel regulator of M2-macrophage polarization: Sustains STAT6 phosphorylation by directly binding and inhibiting tyrosine phosphatase SHP-1.
    Article Snippet: Identifying novel factors driving M2-macrophage polarization may help understand immune modulation in cancers.. We identified DARPP-32, a protein phosphatase inhibitor, to be significantly upregulated in FBXW7 knock out THP-1 spontaneously polarized to M2 macrophages.. DARPP-32 levels remarkably increased in macrophages treated with different M2-inducers while it copiously decreased upon treatment with M1 inducer Lipopolysaccharide/IFN-γ.

    Article Title: β-Arrestin 1 Differentially Modulates cAMP and ERK Pathways Downstream of the FSH Receptor
    Article Snippet: Standard curves were generated to calculate the cAMP concentrations using GraphPad Prism software (version 6.0). .. To generate a stable β-arrestin 1 KO cell line via HDR, HEK 293 cells were co-transfected with a CRISPR/Cas9 KO plasmid and β-arrestin 1 HDR template plasmid (Santa Cruz Biotechnology, Dallas, TX, USA). ..

    Article Title: Placental Insulin-like Growth Factor 1 Deficiency Drives Autism-Relevant Behavioral Changes with Sex-Specific Vulnerabilities
    Article Snippet: .. IGF-I CRISPR/Cas9 KO Plasmid (m) and Control CRISPR/Cas9 plasmids (Santa Cruz Biotechnology) were diluted to 0.073 μg/μl. ..

    Article Title: Lipid transfer by ORP3 is required for the regulation of PI4P and PI(4,5)P2 at the plasma membrane in mitosis
    Article Snippet: .. The HeLa AP CRISPR-Cas9 ORP3-KO cell line was generated using a GFP single nickase ORP-3 CRISPR/Cas9 KO Plasmid (Santa Cruz), containing a pool of the three following guide RNA (gRNAs): These gRNA are targeting sequences in the mRNA sequence of ORP3 prior to the start codon of the wild type isoform, which are absent from the sequences of ORP3 plasmids used for rescue experiments. ..

    Article Title: Genome-wide CRISPR screens identify the EXO1-CAF-1 pathway suppressing R-loop-associated DNA damage
    Article Snippet: HeLa cells (obtained from ATCC) were grown in Dulbecco’s modified Eagle’s media. .. To knock-out EXO1 in HeLa cells, a commercially available CRISPR/Cas9 KO plasmid (Santa Cruz Biotechnology sc-402356) was used, as we previously described [ ]. .. Transfected cells were FACS-sorted into 96-well plates using a BD FACSAria II instrument.

    Article Title: Genome-wide CRISPR screens identify the EXO1-CAF-1 pathway suppressing R-loop-associated DNA damage.
    Article Snippet: Cell culture and protein techniques HeLa cells (obtained from ATCC) were grown in Dulbecco’s modified Eagle’s media. .. To knock-out EXO1 in HeLa cells, a commercially available CRISPR/Cas9 KO plasmid (Santa Cruz Biotechnology sc-402356) was used, as we previously described [ 23 ]. .. Cell culture and protein techniques HeLa cells (obtained from ATCC) were grown in Dulbecco’s modified Eagle’s media.

    Article Title: Human RIG-I deficiency confers susceptibility to Kaposi Sarcoma via loss of latency control
    Article Snippet: .. To generate RIG-I–deficient fibroblasts, cells were co-transfected with the human RIG-I CRISPR/Cas9 KO Plasmid (Santa Cruz Biotechnology, sc-400812) and the corresponding homology-directed repair (HDR) plasmid (sc-400812-HDR), using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s protocol. ..

    Plasmid Preparation:

    Article Title: Compounds, compositions, methods of using, and methods for preparing compounds
    Article Snippet: .. At 60% confluency cells, cell were co-transfected with 2 μg each of CRISPR/Cas9 KO Plasmid (sc-400511-KO-2; Santa cruz) and HDR Plasmid (sc-400511-HDR-2; Santa cruz) using UltraCruz® Transfection Reagent (sc-395739; Santa Cruz). .. The double positive cells for GFP and RFP were sorted using MoFlo XDP sorting instrument (Beckman Coulter).

    Article Title: DARPP-32 is a novel regulator of M2-macrophage polarization: Sustains STAT6 phosphorylation by directly binding and inhibiting tyrosine phosphatase SHP-1.
    Article Snippet: Identifying novel factors driving M2-macrophage polarization may help understand immune modulation in cancers.. We identified DARPP-32, a protein phosphatase inhibitor, to be significantly upregulated in FBXW7 knock out THP-1 spontaneously polarized to M2 macrophages.. DARPP-32 levels remarkably increased in macrophages treated with different M2-inducers while it copiously decreased upon treatment with M1 inducer Lipopolysaccharide/IFN-γ.

    Article Title: β-Arrestin 1 Differentially Modulates cAMP and ERK Pathways Downstream of the FSH Receptor
    Article Snippet: Standard curves were generated to calculate the cAMP concentrations using GraphPad Prism software (version 6.0). .. To generate a stable β-arrestin 1 KO cell line via HDR, HEK 293 cells were co-transfected with a CRISPR/Cas9 KO plasmid and β-arrestin 1 HDR template plasmid (Santa Cruz Biotechnology, Dallas, TX, USA). ..

    Article Title: Placental Insulin-like Growth Factor 1 Deficiency Drives Autism-Relevant Behavioral Changes with Sex-Specific Vulnerabilities
    Article Snippet: .. IGF-I CRISPR/Cas9 KO Plasmid (m) and Control CRISPR/Cas9 plasmids (Santa Cruz Biotechnology) were diluted to 0.073 μg/μl. ..

    Article Title: Lipid transfer by ORP3 is required for the regulation of PI4P and PI(4,5)P2 at the plasma membrane in mitosis
    Article Snippet: .. The HeLa AP CRISPR-Cas9 ORP3-KO cell line was generated using a GFP single nickase ORP-3 CRISPR/Cas9 KO Plasmid (Santa Cruz), containing a pool of the three following guide RNA (gRNAs): These gRNA are targeting sequences in the mRNA sequence of ORP3 prior to the start codon of the wild type isoform, which are absent from the sequences of ORP3 plasmids used for rescue experiments. ..

    Article Title: Genome-wide CRISPR screens identify the EXO1-CAF-1 pathway suppressing R-loop-associated DNA damage
    Article Snippet: HeLa cells (obtained from ATCC) were grown in Dulbecco’s modified Eagle’s media. .. To knock-out EXO1 in HeLa cells, a commercially available CRISPR/Cas9 KO plasmid (Santa Cruz Biotechnology sc-402356) was used, as we previously described [ ]. .. Transfected cells were FACS-sorted into 96-well plates using a BD FACSAria II instrument.

    Article Title: Genome-wide CRISPR screens identify the EXO1-CAF-1 pathway suppressing R-loop-associated DNA damage.
    Article Snippet: Cell culture and protein techniques HeLa cells (obtained from ATCC) were grown in Dulbecco’s modified Eagle’s media. .. To knock-out EXO1 in HeLa cells, a commercially available CRISPR/Cas9 KO plasmid (Santa Cruz Biotechnology sc-402356) was used, as we previously described [ 23 ]. .. Cell culture and protein techniques HeLa cells (obtained from ATCC) were grown in Dulbecco’s modified Eagle’s media.

    Article Title: Human RIG-I deficiency confers susceptibility to Kaposi Sarcoma via loss of latency control
    Article Snippet: .. To generate RIG-I–deficient fibroblasts, cells were co-transfected with the human RIG-I CRISPR/Cas9 KO Plasmid (Santa Cruz Biotechnology, sc-400812) and the corresponding homology-directed repair (HDR) plasmid (sc-400812-HDR), using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s protocol. ..

    Transfection:

    Article Title: Compounds, compositions, methods of using, and methods for preparing compounds
    Article Snippet: .. At 60% confluency cells, cell were co-transfected with 2 μg each of CRISPR/Cas9 KO Plasmid (sc-400511-KO-2; Santa cruz) and HDR Plasmid (sc-400511-HDR-2; Santa cruz) using UltraCruz® Transfection Reagent (sc-395739; Santa Cruz). .. The double positive cells for GFP and RFP were sorted using MoFlo XDP sorting instrument (Beckman Coulter).

    shRNA:

    Article Title: DARPP-32 is a novel regulator of M2-macrophage polarization: Sustains STAT6 phosphorylation by directly binding and inhibiting tyrosine phosphatase SHP-1.
    Article Snippet: Identifying novel factors driving M2-macrophage polarization may help understand immune modulation in cancers.. We identified DARPP-32, a protein phosphatase inhibitor, to be significantly upregulated in FBXW7 knock out THP-1 spontaneously polarized to M2 macrophages.. DARPP-32 levels remarkably increased in macrophages treated with different M2-inducers while it copiously decreased upon treatment with M1 inducer Lipopolysaccharide/IFN-γ.

    Control:

    Article Title: Placental Insulin-like Growth Factor 1 Deficiency Drives Autism-Relevant Behavioral Changes with Sex-Specific Vulnerabilities
    Article Snippet: .. IGF-I CRISPR/Cas9 KO Plasmid (m) and Control CRISPR/Cas9 plasmids (Santa Cruz Biotechnology) were diluted to 0.073 μg/μl. ..

    Generated:

    Article Title: Lipid transfer by ORP3 is required for the regulation of PI4P and PI(4,5)P2 at the plasma membrane in mitosis
    Article Snippet: .. The HeLa AP CRISPR-Cas9 ORP3-KO cell line was generated using a GFP single nickase ORP-3 CRISPR/Cas9 KO Plasmid (Santa Cruz), containing a pool of the three following guide RNA (gRNAs): These gRNA are targeting sequences in the mRNA sequence of ORP3 prior to the start codon of the wild type isoform, which are absent from the sequences of ORP3 plasmids used for rescue experiments. ..

    Sequencing:

    Article Title: Lipid transfer by ORP3 is required for the regulation of PI4P and PI(4,5)P2 at the plasma membrane in mitosis
    Article Snippet: .. The HeLa AP CRISPR-Cas9 ORP3-KO cell line was generated using a GFP single nickase ORP-3 CRISPR/Cas9 KO Plasmid (Santa Cruz), containing a pool of the three following guide RNA (gRNAs): These gRNA are targeting sequences in the mRNA sequence of ORP3 prior to the start codon of the wild type isoform, which are absent from the sequences of ORP3 plasmids used for rescue experiments. ..

    Knock-Out:

    Article Title: Genome-wide CRISPR screens identify the EXO1-CAF-1 pathway suppressing R-loop-associated DNA damage
    Article Snippet: HeLa cells (obtained from ATCC) were grown in Dulbecco’s modified Eagle’s media. .. To knock-out EXO1 in HeLa cells, a commercially available CRISPR/Cas9 KO plasmid (Santa Cruz Biotechnology sc-402356) was used, as we previously described [ ]. .. Transfected cells were FACS-sorted into 96-well plates using a BD FACSAria II instrument.

    Article Title: Genome-wide CRISPR screens identify the EXO1-CAF-1 pathway suppressing R-loop-associated DNA damage.
    Article Snippet: Cell culture and protein techniques HeLa cells (obtained from ATCC) were grown in Dulbecco’s modified Eagle’s media. .. To knock-out EXO1 in HeLa cells, a commercially available CRISPR/Cas9 KO plasmid (Santa Cruz Biotechnology sc-402356) was used, as we previously described [ 23 ]. .. Cell culture and protein techniques HeLa cells (obtained from ATCC) were grown in Dulbecco’s modified Eagle’s media.



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